Angiotensin Converting Enzyme, Bovine (ACE)
754.25 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-A2295-01A
Supplier
US Biologicals
Size
1U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | ACE (kininase II, EC 3.4.15.1) is a zinc dipeptidyl carboxypeptidase (1). ACE catalyses both angiotensin I transformation to angiotensis II (a potent vasopressor agent) and degradation of bradikinin, a vasodepressor. Thus, ACE appears to play a key role in regulating vascular tone and remodeling as well as in the development of atherosclerotic lesions. Increasing evidence asuggests that ACE plays an important role in vascular pathology. Recent studies show two new functions for this important enzyme: 1) ACE enhances presentation of endogenous antigens (such as HIV gp160-derived peptide p18) to MHC class I-restricted T-lymphocytes (1); 2) amino-terminal domain of ACE efficiently cleaves hemoregulatory tetrapeptide N-Ac-Ser-Asp-Lys-Pro (2,3) which is involved in the control of haemopoetic stem cell proliferation (the amount of this peptide greatly decreases in patients undergoing cancer chemotherapy) (4). Specific Activity: ~15mU/ug of protein. Unit Definition: One unit produces 1umole of hippuric acid or His-Leu from Hippuril-His-Leu per min. in 0.1M phosphate buffer, 300mM sodium chloride, pH 8.3 at 37°C. Inhibitors: Metalloproteinase inhibitors such as EDTA and 1,10-phenanthroline. Stable pH: 7.5-8.5. Applications: Suitable for use as a reference in SDS-PAGE, Western Blot (Use 20ng/lane) and Immunoprecipitation. Also suitable for enzymatic studies including substrate specificity and enzyme kinetics. Other applications not tested. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Bovine lung membrane |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ~95% (SDS-PAGE) |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.2mM CHAPS. No preservatives added. Stable at pH 7.5-8.5. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
