Anhydrotrypsin, Bovine
786.79 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-T8656-20
Supplier
US Biologicals
Size
100 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Treatment of trypsin with phenylmethylsulfonyl fluoride (PMSF) followed by a strong base leads to the conversion of Serine 195 to dehydroalanine at the active site (1). Alpha and beta forms of anhydrotrypsin are separated by immobilized soybean trypsin inhibitor. The activity of anhydrotrypsin is greatly reduced compared to native trypsin as quantified by chromogenic substrate (2). Anhydrotrypsin provided by Molecular Innovations is the single chain beta-form prepared from Type XIII bovine trypsin. It has a MW of 23.3kD and an extinction coefficient of 1.54mg-1 ml-1. Source: Anhydrotrypsin, from bovine pancreas Molecular Weight: 83kD Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Source Antigen | Bovine pancreas |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 83 |
Product Properties
| Storage | -70°C |
| Grade | Highly Purified |
| Purity | ~95% (SDS-PAGE) |
| Form | Supplied as a liquid in 1mM HCl, 10mM CaCl2. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
