BpiI (BbvII)
428.67 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-B2640
Supplier
US Biologicals
Size
200U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | 5′-G A A G A C (N)2∨-3′ 3′-C T T C T G (N)6^-5′ Supplied With: R1625: Restriction Enzyme Buffer A, 10X, Dilute to 1X. 1X buffer compostion is 33mM Tris-acetate pH 7.9 at 37°C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. R1625-02: Restriction Enzyme Buffer C, 10X, Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl pH 7.5, 10mM MgCl2, 50mM sodium chloride, 0.1mg/ml BSA. Unit Definition: One unit is defined as the amount of BpiI required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of reaction buffer. Diluent Buffer: 10mM Tris-HCl, pH 7.4 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Storage Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Enzyme Properties: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BpiI. Ligation/Recutting Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of single-stranded or double-stranded labeled oligonucleotide occurred duringincubation with 10 units of Bpil for 4 hours. Blue/White Cloning Assay: The B/W assay was relpaced with L0 test after validating experiments showed L0 test ability to detect nuclease and phosphatase activities with sensitivity that equals to that of B/W test. Methylation Effects: Dam, Dcm, EcoKl: never overlaps – no effect CpG: may overlap – no effect EcoBl: may overlap – effect not determined Stability during Prolonged Incubation: A minimum of 0.3units of BpiI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. Thermal Inactivation: BpiI is inactivated by incubation at 65°C for 20 minutes. Digestion of Agarose-embedded DNA: A minimum of 5units of BpiI is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Number of Recognition Sites in DNA: Lambda: 24 PhiX174: 3 pBR322: 3 pUC57: 0 pUC18/19: 0 pTZ19R/U: 0 M13mp18/19: 0 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Bacillus pumillus Sw 4-3 |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
