Bsp119I (AsuII)
429.25 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-B2940
Supplier
US Biologicals
Size
2500U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Sequence: 5′-T T^C G A A-3′ 3′-A A G C^T T-5′ Concentration: 10u/ul Source: Bacillus species RFL119 Storage Buffer: Supplied as a liquid in10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Suplied as a liquid in 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Enzyme Properties Compatible Ends: Bsu15I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp11406I, Ssil, TaqI, XmiI Methylation Effects: Dam, Dcm, EcoBI: Never overlaps – No effect. CpG: Completely overlaps – Blocked. EcoKI: May overlap – Effect not determined. Stability during Prolonged Incubation: A minimum of 0.1units of Bsp119I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. Thermal Inactivation: Bsp119I is inactivated by incubation at 80°C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5units of Bsp119I is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Number of Recognition Sites in DNA: Lambda: 7 PhiX174, M13mp18/19, pBR322, pUC18/19, pUC57, pTZ19R/U, pTZ57R, pBluescriptIIKS(+/-), pBluescriptIISK(+/-): 0 pACYC177: 1 pACYC184: 2 |
| Source Antigen | |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
