BsuRI (HaeIII)
430.68 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-B3100
Supplier
US Biologicals
Size
3000U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | 5′-G G^C C-3′ 3′-C C^G G-5′ Concentration: 10u/ul Source: Bacillus subtilis R Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Thermal Inactivation: BsuRI is inactivated by incubation at 80°C for 20 minutes. Storage Buffer: 10mM Tris-HCl, pH 7.5, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Supplied with: R1625-Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate (pH 7.9 at 37°C), 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. R1625-04: Dilute to 1X for 100% BsuRI digestion. 10mM Tris-HCl, pH 8.5, 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 37°C Incubation Temperature: 37°C Unit Definition: One unit is defined as the amount of BsuRI required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. Methylation Effects: Dam, EcoKl,EcoBl: never overlaps – no effect. Dcm, CpG: may overlap – no effect. Protocol for Digestion: Add: Nuclease free water: 16ul R1625-04: 2ul DNA (0.5-1ug/ml): 1ul B3100: 0.5-2ul Mix gently and spin down for a few seconds. Incubate at 37ºC for 1-16 hours. Protocol for Digestion Directly after Amplification: Add: PCR Reaction Mixture: 10ul (~0.1-0.5ug of DNA) Nuclease free water: 18ul R1625-04: 2ul B3100: 1-2ul Mix gently and spin down for a few seconds. Incubate at 37ºC for 1-16 hours. Storage and Stability: Aliquot and store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Bacillus subtilis R |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
