Calpain, mu
554.08 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-C1035-33M
Supplier
US Biologicals
Size
2 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | This protein consists of an 80kD large subunit and a 30kD small subunit. The protein was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns. Applications: This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption we suggest the following procedure: A- Dilute 1µl of the antiserum against µ-calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1000). B- Add 1µg of protein to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. E-Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented. Stability: m-Calpain although stable at 100C for 1 week, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
| Source Antigen | Bovine Myocardium |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ≥ 90% as determined by reducing and non-reducing SDS-PAGE Coomassie. |
| Form | Supplied as a liquid in 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT and 50% glycerol, pH 7.4. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
