Cas9 (D10A) Nickase, Nuclear Localization Sequence (NLS)
471.55 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-506201
Supplier
US Biologicals
Size
10 µg (1 mg/ml)
Product Overview
Product Properties
Target Gene
Product Overview
| Description | NLS-Cas9-D10A Nickase is a mutation form of Cas9 Nuclease. Cas9 nuclease is an RNA-guided endonuclease that can catalyze cleavage of double stranded DNA. This kind of targeted nuclease is a powerful tool for genome editing with high precision. Cas9 nuclease cleaves the double strand DNA generating two break sites based on its two active domains. NLS-Cas9-D10A Nickase is a mutation form of Cas9 Nuclease which makes one active domain deactivated, thus it can only cut one single strand DNA that is complementary to the guide-RNA, producing one single strand cut. Combined with two different gRNA, NLS-Cas9-D10A Nickase produces two cut sites respectively and causes a double strand break. Compared with the wild type Cas9 nuclease, the two-gRNA guided cleavage can significantly reduce the off target effects. NLS-Cas9-D10A Nickase is highly purified mutant proteins expressed in an E. coli strain carrying a plasmid encoding the mutated Cas9 gene from Streptococcus pyogenes. Applications: Screening the highly efficient and specific targeting gRNAs using in vitro DNA cleavage. In vivo gene editing combined with specific gRNA by electroporation or injection. Key Features: DNA-free: no external DNA added to system. High cleavage efficiency: NLS ensures the efficient entry of Cas9 protein into nuclei. Low off target: Double gRNA-guided cleavage and transient expression of Cas9 nuclease. Time-saving: no need for transcription and translation. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Source Antigen | E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | Supplied as 10mM Tris, pH 7.4, 300mM sodium chloride, 0.1mM EDTA, 1mM DTT, 50% glycerol. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
