Caspase 1, Active, Recombinant, Human
539.97 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-C2086-66
Supplier
US Biologicals
Size
25u
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Caspase 1 (also know as ICE, Interleukin-1b-Converting Enzyme) is the prototypical member of the caspase family of cysteine proteases. Caspase 1 exists in cells as an inactive 45kD pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20kD) and small (10kD) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of Caspase 1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the Caspase 1 is present in the inactive pro-enzyme form. The active Caspase 1 preferentially cleaves Caspase 1 substrates (e.g., YVAD-AFC or YVAD-pNA). The active recombinant Caspase 1 is routinely tested for its ability to enzymatically cleave these two substrates. Active recombinant human Caspase 1, corresponding to aa120-404, N-terminal His-tag, was expressed in E. coli (NP_150634.1) Related Products: C2086-67: Caspase 1, Fluorometric Substrate, YVAD-AFC (Ac-Tyr-Val-Ala-Asp-AFC) C2086-68: Caspase 1 (ICE) Colorimetric Substrate, YVAD-pNA Specific Activity: ~5000 units/mg Unit Definition: One unit of the recombinant Caspase 1 is the enzyme activity that cleaves 1nmol of the caspase substrate YVAD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50mM Hepes, pH 7.2, 50mM sodium chloride, 0.1% Chaps, 10mM EDTA, 5% glycerol and 10mM DTT. Applications: Suitable for use in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in Caspase 1 activity assays. Other applications not tested. Recommended Dilutions: Analyzing Caspase Activity: 1 unit per assay Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 12 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -70°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | Supplied as a lyophilized powder. Reconstitute to 1 unit per ul in sterile PBS. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
