Caspase 10a, active, Recombinant, Human (FLICE2, Mch4)
498.93 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-C2088-42
Supplier
US Biologicals
Size
25U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Caspase-10a (also know as Mch4) is a member of the interleukin-1b converting enzyme (ICE) family of cysteine proteases. Similar as other caspases, caspase-10 exists in cells as an inactive proenzyme. During apoptosis, procaspase-10 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed form of caspase-10 consists of large (18kD) and small (11kD) subunits which associate to form the active enzyme. Recombinant Human Caspase-10a was expressed in E. coli and routinely tested for its ability to enzymatically cleave the colorimetric substrate Ac-IETD-pNA. Useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate, or as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity. Unit Definition: One unit of the recombinant caspase-10a is the enzyme activity that cleaves 1nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50mM Hepes, pH 7.2, 50mM NaCl, 0.1% Chaps, 10mM EDTA, 5% Glycerol, and 10mM DTT. Form: Lyophilized Specific Activity: 8,000 units/mg Reconstitution: Reconstitute to 1 unit per ml of PBS. Storage: The lyophilized caspase-10a is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -70°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
