Caspase 3, active, Recombinant, Human (CPP-32, Apoptain, Yama, SCA-1)
469.24 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-C2087-13
Supplier
US Biologicals
Size
25u
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Caspase-3 (also know as CPP32, Yama and apopain) is a member of the interleukin-1b converting enzyme (ICE) family of cysteine proteases. Caspase-3 exists in cells as an inactive 32kD proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The active recombinant caspase-3 is routinely tested for its ability to enzymatically cleave the caspase-3 colorimetric substrate Ac-DEVD-pNA. The recombinant active human caspase-3 expressed in E. coli and fused with His tag, spontaneously undergoes auto processing to yield subunits characteristic of the native enzyme (full length protein Accession No. NP_004337). The recombinant caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA). In combination with caspase-3 activity assays, the active recombinant caspase-3 is very useful in screening of caspase inhibitors for pharmaceutical applications. The recombinant enzyme can also be used as a positive control in caspase assays or in determining the specificity of caspase substrates. For a complete caspase-3 assay protocol, please refer to C2087-10 Caspase-3/CPP32 Fluorometric or C2087-20 Colorimetric Assay Kits. Specific Activity: ≥15,000 units/mg Unit Definition: One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50mM Hepes, pH 7.2, 50mM sodium chloride, 0.1% Chaps, 10mM EDTA, 10mM DTT, 5% glycerol. Applications: Suitable for use in Caspase 3 activity assays, studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase assays. Other applications not have not been tested. Recommended Dilutions: Caspase-3 activity assay: Use 1unit/assay for analyzing caspase assay. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -70°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to 1unit/ul. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
