Caspase 3 Fluorometric Substrate, DEVD (CPP-32, Apoptain, Yama, SCA-1)
554.07 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-C2087-11
Supplier
US Biologicals
Size
200Tests
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Fluorometric substrate for Caspase-3. N-acetyl-Asp-Glu-Val-Asp-AFC; 7-amino-4-trifluoromethyl coumarin Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Used in the BioAssay™ Caspase-3 Apoptosis Detection Kit (Cat.# C2087-10), providing a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7uM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC (7-amino-4-trifluoromethyl coumarin) at Ex 400nm and Em 505nm, using a fluorometer or a multi-well fluorescence plate reader. Upon proteolytic cleavage of the substrate by caspases, free AFC emits a yellow-green fluorescence. The increase in the yellow-green fluorescence is proportional to the increase in caspase activity.Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. Storage and Stability: Store at -20°C, protected from light. 6 months under proper storage conditions |
| Source Antigen | |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 729 |
Product Properties
| Storage | -20°C |
| Grade | |
| Purity | ≥ 98% (HPLC) |
| Form | Supplied as a liquid in 1mM DMSO. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
