Caspase-3, Proform, Recombinant, Mouse (Casp3, Apopain, Cysteine Protease CPP32, CPP-32, LICE, Protein Yama, SREBP Cleavage Activity 1, SCA-1, Cpp32)
655.65 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-169638
Supplier
US Biologicals
Size
5 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Caspase-3 (also know as CPP32, Yama and apopain) is a member of the caspase family of cysteine proteases. Caspase-3 exists in cells as an inactive 32kD proenzyme. Proteolytic processing of this inactive proenzyme generates the 17kD and 12kD subunits which, when assembled as a tetramer (a pair of heterodimers), form the active caspase. Cascades of caspase activation have been shown to be key signal-transducing events in apoptosis. Procaspase-3, like other procaspases with short N-terminal prodomains, is considered to be a downstream or “effector” enzyme. Pro-caspase-3 can be cleaved and activated by caspase-8 and –10 which are themselves activated through interaction with death ligand receptor/adapter protein complexes (e.g., Fas/FADD). Procaspase-3 is also processed by caspase-9, the apical caspase that is activated by binding to the Apaf-1/cytochrome c/ATP “apoptosome”. Thus, activation of procaspase-3 stands at a point of convergence for the two major types of apoptosis signaling pathways-those linked to cell surface death receptors and those linked to mitochondrial release of cytochrome c. The recombinant Procaspase-3 is produced by expression of a human cDNA in E. coli. The purified protein is highly activatable by treatment with active recombinant caspase-8. We routinely test the activation using 10-30ng of the procaspase-3 treated with 0.1-0.5 units of the active recombinant caspase-8 in 100ul of Reaction Buffer for 30 minutes. Activation of procaspase-3 can be monitored using the Caspase-3 Fluorometric and Colorimetric Assay Kits. Source: Recombinant protein corresponding to mouse Caspase-3 Proform, expressed in E. coli. Molecular Weight: ~32kD Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 32 |
Product Properties
| Storage | -70°C |
| Grade | Purified |
| Purity | ~90% (SDS-PAGE) |
| Form | Supplied as a lyophilized powder. Reconstitute with PBS, 15% glycerol to 0.1ug/ul. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
