Caspase Family Group I, Active, Recombinant Set (Caspases-1,4,5)
624.45 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-C2086-10J
Supplier
US Biologicals
Size
3x25U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Caspase family proteases play an important role in apoptosis. Currently, the family consists of more than 10 members. Based on their extended substrate specificities, these enzymes were divided into three major groups: Group I (contains caspase-1,-4,-5) Group II (contains caspase-2,-3,-7) Group III (contains caspase-6,-8,-9,-10) Evidences have suggested that Group I caspases are involved primarily in the production of inflammatory cytokines, while group II and III enzymes function in apoptosis as effectors and upstream activators, respectively. Active recombinant human caspases were expressed in E. coli and routinely tested for their ability to enzymatically cleave the substrate YVAD-pNA (for caspase-1), WEHD-pNA (for caspase-4 & -5), VEID-pNA (for caspase-6), IETD-pNA (for caspase-8 & -10) LEHD-pNA (for caspase-9), respectively. Highly active recombinant human caspases were expressed in E. coli and purified. Active recombinant caspases are useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates and as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity. Kit Components: C2086-10J1: Active Recombinant Caspase-1, 1×25 units C2086-10J2: Active Recombinant Caspase-4, 1×25 units C2086-10J3: Active Recombinant Caspase-5, 1×25 units Form: Lyophilized powder Reconstitution: 1 unit/ul in PBS with 15% glycerol Unit Definition: One unit of the active recombinant caspase-1,-4,-5 is the enzyme activity that cleaves 1nmol of the caspase substrate YVAD-pNA (for caspase-1) and WEHD-pNA (for caspase-4 & -5), respectively, per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% Glycerol, 10mM DTT. Applications: Active recombinant caspases are useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates, and as positive controls in caspase activity assays and Western Blot analysis. We recommend using 1 unit per assay for analyzing caspase activity. Storage and Stability: The lyophilized recombinant caspases are stable for 6 months after receipt at -70°C. Following reconstitution in PBS, 15% glycerol, these enzymes should be aliquoted and stored at -70°C. Avoid multiple freeze/thaw cycles as activity might decrease. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -70°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
