Cathepsin S, Recombinant, Human, aa115-331, Active (CTSS)
583.66 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-301190
Supplier
US Biologicals
Size
10 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Cathepsin S (CTSS) is a lysosomal cysteine protease of the papain family and may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. CTSS is synthesized as inactive precursor of 331aa consisting of a 15aa signal peptide, a propeptide of 99aa, and a mature polypeptide of 217aa. It is activated in the lysosomes by a proteolytic cleavage of the propeptide. The deduced amino acid sequence contains only one potential N-glycosylation site located in the propeptide. Compared with the abundant cathepsins B, L and H, cathepsin S shows a restricted tissue distribution, with highest levels in spleen, heart, and lung. In addition, evidences indicate that cathepsin S generates amyloid beta-peptide from amyloidogenic fragments of amyloid precursor protein (APP) in the endosomal/lysosomal compartment, and is implicated in the pathogenesis of Alzheimer’s disease and Down Syndrome. Source: Recombinant protein corresponding to aa115-331 human Cathepsin S, expressed in E. coli. Molecular Weight: ~23.9kD Biological Activity: Active Cathepsin S has been prepared by auto-catalytic activation of Procathepsin S at low pH. The enzyme is fully active as seen from its ability to cleave a fluorogenic substrate Z-VVR-AFC. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt at -70°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 23.9 |
Product Properties
| Storage | -70°C |
| Grade | Highly Purified |
| Purity | ~90% (SDS-PAGE) |
| Form | Supplied as a lyophilized powder. Reconstitute with 50mM sodium acetate, 100mM sodium chloride, pH 5.5. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
