CDw124, Recombinant, Human (Interleukin 4 Receptor alpha, Soluble, IL-4Ra)
396.23 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-C2587-60A
Supplier
US Biologicals
Size
3 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Human IL-4 exerts its biological effects via signaling through its receptor system, IL-4R. There are two types of the IL-4 receptor complexes, the type I receptor consisting of the IL-4R alpha and the common gamma chain, yc and the type II receptor consisting of IL-4R alpha and the IL-13R alpha. The IL-4R alpha chain contains an extracellular domain of 207 amino acids, a transmembrane domain of 24 amino acids, and an intracellular domain of 569 amino acids. The secreted extracellular domain, sIL-4R alpha, also called CD124 is capable of blocking IL-4 activities. This receptor plays a major role in the regulation of the differentiation of naive CD4+ T cells and class switching to IgG1 and IgE. Recombinant human sIL-4Rα is a 209 amino acid protein which corresponds to the entire extracellular domain of IL-4Rα. AA Sequence: GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH Biological Activity: The ED50 as determined by its ability to inhibit the IL-4 dependent proliferation of human TF-1 cells is < 5ng/ml (in the presence of 0.5ng/ml IL-4), corresponding to a specific activity of ≥ 2x10e5 units/mg. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, A293 cells |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ≥ 97% by SDS-PAGE and HPLC analyses. Endotoxin: ≤0.1ng/ug (1EU/ug) |
| Form | Supplied as a lyophilized powder from PBS, pH 7.0. Reconstitution in ddH2O to 0.1-1mg/ml. This solution can then be diluted into other aqueous buffers. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
