CENP-B, Recombinant, Human (Centromere Protein B)
421.96 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-C2615-12H
Supplier
US Biologicals
Size
5 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENP-A to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies. CENP-B is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66kD and plays an important role in the formation of the centromeric chromatin. CENP-B antibodies are present in the sera of up to 80% of patients with CREST syndrome. Recombinant Human CENP-B produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 80kD.. Recombinant human CENP-B is expressed with a -6X His Tag and purified by proprietary chromatographic techniques. Immunological Functions: 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera). Applications: Suitable for use in ELISA, Western Blot, for biotinylation and iodination.. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, Sf9 insect cells |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 80 |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ≥95% (SDS-PAGE) |
| Form | Supplied as a liquid in 20mM HEPES buffer, pH 7.6, 250mM sodium chloride and 20% glycerol. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
