Cfr10I (BsrFI)
406.74 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-C3275
Supplier
US Biologicals
Size
200U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Sequence: 5′- R ^C C G G Y -3′ 3′- Y G G C C^ R -5′ Source: E.coli that carries the cloned cfr10IR gene from Citrobacter freundii RFL10 Concentration: 10 units/ul Unit Definition: One unit is defined as the amount of Cfr10I required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. Incubation Temperature: 37°C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: Supplied as a liquid in 10mM potassium phosphate (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Supplied with: R1625-45: Restriction Enzyme Buffer for Cfr10I, 10X: Supplied as a liquid in 100mM Tris-HCl, pH7.5, 50mM magnesium chloride, 1M sodium chloride, 10mg/ml BSA, 0.2% Triton X-100. R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 330mM Tris-acetate, pH 7.9, 100mM magnesium acetate, 660mM potassium acetate, 1mg/ml BSA. Enzyme Properties: Star Activity: An excess of Cfr10I (10u/ug DNA x 1 hour) or low salt concentration may result in star activity. Compatible Ends: BshTI, BsaWI, Cfr9I, Eco88I, Kpn2I, MreI, NgoMIV, SgrAI. Methylation Effects: Dam, Dcm: Never overlaps – No effect. CpG: Completely overlaps – Blocked. EcoKI, EcoBI: May overlap – No effect. Stability during Prolonged Incubation: A minimum of 0.1units of Cfr10I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. Digestion of Agarose-embedded DNA: A minimum of 5units of Cfr10I is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Number of Recognition Sites in DNA: Lambda: 61 PhiX174: 0 M13mp18/19, pUC18/19, pUC57: 1 pTZ19R/U: 2 pBR322: 7 Note: For cleavage with Cfr10I, at least two copies of its recognition sequence are required. Thermal Inactivation: Cfr10I is not inactivated by incubation at 80°C for 20min (see Protocol for Inactivation Procedure). |
| Source Antigen | |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
