Collagen Type I, Bovine (FITC)
598.89 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-C7510-11
Supplier
US Biologicals
Size
10 mg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | FITC-labeled bovine type I is an excellent substrate for examining collagenase activity. This labeled substrate is highly purified and telo-peptide free. To minimize background levels in collagenase assays, FITC-labeled collagen has been enzymatically pre-treated and further purified by ion-exchange chromatography. Applications: Suitable for use in the assay of mammalian collagenase activity. Solubility: FITC-bovine type I collagen is soluble in acidic buffer (maximum 4mg/ml), but difficult to dissolve in neutral buffer. In order to dissolve in neutral buffer, dissolve in 0.01M acetic acid at 4mg/ml, and then add into neutral buffer (final NaCl concentration should be 0.15-0.2M). FITC-bovine type I collagen forms stable gel in neutral buffer and can be used as a substrate for assaying collagenase activity by collagen-gel method. Storage and Stability: Store at -20ºC in the dark. The collagen solution is stable in acidic solution at -20ºC but will gradually degrade under neutral conditions. Collagen will form insoluble gel and degrade under neutral conditions during long-term storage at 4°C. |
| Source Antigen | Bovine skin |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 300 |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | ≥99% by SDS-PAGE. Background value is less than 0.1% (free fluorescence intensity (FI) extracted with 35% ethanol at 25°C). |
| Form | Suppllied as liquid in 0.01M acetic acid. Labeled with FITC. Molar ratio: 1:1 |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
