Collagen Type II, Bovine (FITC)
383.11 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-C7510-37
Supplier
US Biologicals
Size
1 mg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. FITC-labeled bovine type II is an excellent substrate for examining collagenase activity. This labeled substrate is highly purified and telo-peptide free and is supplied lyophilized or in solution. To minimize background levels in collagenase assays, FITC-labeled collagen has been enzymatically pre-treated and further purified by ion-exchange chromatography. FITC-labeled collagen can also be used as a substrate for cell culture. Collagen degradation products in the culture supernatants can be directly determined by measured the flourescence at 520nm (Emission)/490 nm (Excitation). Molar Ratio: ~1:1-Collagen: FITC Applications: Suitable for use in assaying mammalian collagenase activity. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Bovine articular cartilage |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 300 |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | ≥ 99% by SDS-PAGE |
| Form | Supplied as a liquid in 0.01M acetic acid. Labeled with FITC. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
