Collagenase, Type II, C. histolyticum
350.61 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-C7511-30
Supplier
US Biologicals
Size
100 mg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Collagenases degrade native helical collagen fibrils. The substrate, collagen, is the major fibrous component of animal extracellular connective tissue: skin, tendon, blood vessels, bone, etc. Suitable for use in cell dissociation procedures. Collagenase is prepared to contain higher clostripain activity levels. Suitable for use in heart, bone, thyroid, salivary and liver primary cell isolation. Collagenase nomenclature: Type 1 Containing average amounts of assayed activities (collagenase, caseinase, clostripain and tryptic activities). It is generally recommended for fat, adrenal, and liver cells. Type 2 Containing greater clostripain activity. It is generally used for heart, bone, muscle, thyroid, cartilage, and liver cells. Type 3 Containing low proteolytic activity. It is usually used for mammary and fetal cells. Type 4 Containing low tryptic activity. It is commonly used for islets and other applications where receptor integrity is crucial. Activity: ≥125units/mg ≥200 Caseinase units/mg dry weight ≥3.5 Clostripain units/mg dry weight ≥0.1 Tryptic units/mg dry weight A280 @ mg/ml Unit Definition: 1unit releases 1umole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5. Storage and Stability: Lyophilized powder may be stored at 4°C (dessicated). |
| Source Antigen | Clostridium histolyticum |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | 4°C |
| Grade | Molecular Biology Grade |
| Purity | Activity: ≥125units/mg |
| Form | Supplied as a dialyzed, lyophilized powder. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
