Complement C5a, Recombinant, Mouse, His-Tag (C5a, Complement Component 5a, Complement Fragment C5a, Complement-Derived C5a, Complement C5a Protein)
547.67 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-219665
Supplier
US Biologicals
Size
20 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Mouse complement C5a is a 77aa peptide generated during complement activation from the alpha-chain of complement C5. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells. Source: Recombinant protein corresponding to mouse Complement C5a, fused to His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~9kD Biological Activity: The ED50 is 1-3nM, as determined by enzyme release assay of myeloperoxidase Endotoxin: <0.1ng/ug Specificity: Mouse C5a shares 60% and 82% aa sequence identity to human and rat C5a, respectively. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile dH2O, BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 9 |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ~95% (SDS-PAGE) |
| Form | Supplied as a lyophilized powder. No preservative added. Reconstitute with ddH2O, 2.5mg/ml BSA. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
