CUL2, GST-Tag, Recombinant (MGC131970, Cullin-2)
540.31 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-500686
Supplier
US Biologicals
Size
200 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). Source: Partial length protein corresponding to of CUL2, fused to GST-Tag at N-terminal, expressed in E. coli. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Molecular Weight: ~47kD Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | E. coli, Recombinant |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 47 |
Product Properties
| Storage | -20°C |
| Grade | Lysate |
| Purity | Lysate |
| Form | Supplied as a lyophilized powder in Tris-HCl, pH 6.8, 0.2M DTT, 4% SDS, 0.2% bromophenol blue, 20% sucrose. Reconstitute with 200ul sterile dH2O. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
