Deoxyribonuclease I (DNase I), 2u/ul, Solution
379.65 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-D3200-02
Supplier
US Biologicals
Size
100U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Endonuclease which digests dsDNA by hydrolysis of deoxyribonucleotide linkages, producing 3’-hydroxyl oligonucleotides. Applications: Nick Translation DNase I DNA Footprinting Cloning of Random DNA Fragments Activity: ~2000u/ml Unit Definition: 1 unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25ºC when acting upon highly polymerized DNA at pH 5.0. Assay Method: Based on the Kunitz method where DNase will depolymerize DNA; 1 Kunitz units digests 1ug of lambda DNA in 10 minutes at 37°C in 50mM Tris, 1mM Ca+2 and 1mM Mg+2, pH 7.8 in a 50ul reaction. Correlation of digestion units with Kunitz is different for other DNA and buffer systems Quality Control: SDS-PAGE RNase: None Detected Protease: None Detected Storage and Stability: May be stored at 4°C for short-term only. Protect from moisture. For long-term storage, store at -20°C. Aliquots with 50% glycerol are stable for 6 months at -20°C. Note: Pancreatic DNase is highly susceptible to denaturation. Mix by gentle inversion. |
| Source Antigen | Bovine pancreas |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | Chromatographically purified |
| Form | Supplied as a liquid in 50% glycerol, 1mM calcium chloride. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
