DNA Polymerase T4, Exo-Minus
413.83 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-D3943
Supplier
US Biologicals
Size
100U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Catalyzes 5’=>3’ synthesis of DNA. Enzyme has 3’=>5’ exonuclease activity but lacks 5’=>3’ exonuclease activity. T4 DNA polymerase possessing 3’=>5′ exonuclease activity exhibits greater activity on single-stranded DNA than on double-stranded DNA. Source: E. coli with a cloned gene43 of bacteriophage T4. Applications: • Blunting of DNA ends: fill-in 5′-overhangs or/and removal of 3′-overhangs, see protocol • Blunting of PCR products with 3’-dA overhangs. • Synthesis of labeled DNA probes by the replacement reaction. • Oligonucleotide-directed site-specific mutagenesis. • Ligation-independent cloning of PCR products. 5X Reaction Buffer: D3943-05: DNA Polymerase T4 Buffer, 5X: Supplied as a liquid in 335mM Tris-HCl, pH 8.8, 33mM magnesium chloride, 5mM DTT, 84mM (NH4)2SO4. Unit Definition: One unit of T4 DNA Polymerase catalyzes the incorporation of 10nmoles of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30min at 37°C. Activity Assay: Assayed in the following mixture: 67mM Tris-HCl, pH 8.8, 6.7mM MgCl2, 1mM DTT, 16.7mM (NH4)2SO4, 0.2mg/ml BSA, 0.033mM of each dNTP, 0.4mBq/ml [3H]-dTTP, 0.2mM heat-denatured and nuclease-digested calf thymus DNA. Quality Control: No conversion of covalently closed circular DNA to nicked DNA was detected after incubation of 10 units of T4 DNA Polymerase with 1ug of pUC19 DNA in 50ul reaction buffer for 4 hours at 37°C. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | E. coli with a cloned gene43 of bacteriophage T4. |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 104 |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | Supplied as a liquid in 20mM potassium phosphate, pH 7.5, 200mM potassium chloride, 2mM DTT, 50% glycerol. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
