DUSP3, Recombinant, Human, aa1-185, His-Tag (Dual Specificity Protein Phosphatase 3, Dual Specificity Protein Phosphatase VHR, Vaccinia H1-related Phosphatase, VHR)
568.62 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-D9840-06M
Supplier
US Biologicals
Size
100 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DUSP3 is expressed in both breast and ovarian tissues. DUSP3 Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2. Source: Recombinant corresponding to aa1-185 from human DUSP, fused to His-tag at N-terminal expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP Molecular Weight: ~22.6kD (MALDI-TOF) Biological Activity: Determined by measuring the amount of enzyme hydrolyzing 1nm of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH 7.5 using 10mM of substrate, corresponding to a specific activity of >1750IU/mg. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, E.coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 22.6 |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ~95% (SDS-PAGE) |
| Form | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol, 2mM DTT, 0.15M sodium chloride. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
