ECE-1, Recombinant, Mouse (Endothelin Converting Enzyme-1)
761.86 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-E3650-02
Supplier
US Biologicals
Size
10 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Endothelin-converting enzymes (ECEs) hydrolyze a specific peptide bond of big endothelins to produce active endothelins, some of the most potent vasoconstrictors known. ECE-1 is a member of the M13 zinc metallopeptidase family. Other members of the M13 family include thermolysin, neprilysin, Kell , and ECE-2. M13 metallopeptidases can be distinguished from other metallopeptidases by their sensitivity to inhibition by phosphoramidon. ECE-1 is most highly expressed in the cardiovascular endothelium, but is also expressed in some endocrine tissues. ECE-1 is known to hydrolyze a variety of bioactive pepti des, including bradykinin, neurotensin, angiotensins, and Substance P, with a substrate specificity similar to that of neprilysin. ECE-1 displays pronounced pH dependence in its substrate specificity. The degradation of Substance P by ECE-1 in endosomes regulates beta-arrestin-dependent ERK-2 signaling to prevent cell death in some neuronal cells. Four isoforms of ECE-1 are present in humans and mice, all of which encode a Type II integral membrane protein. The four isoforms share a common extracellular catalytic domain, differing in their N-terminal cytoplasmic tail regions. The recombinant mouse ECE-1 transmembrane and cytoplasmic tail domains were replaced with a signal sequence, resulting in the secretion of the soluble catalytic ectodomain. Recombinant protein corresponding to Gln89-Trp769 of mouse ECE-1 with a N-terminal 6-His tag, expressed in CHO cells. UniProt Accession: Q4PZA2. SDS-PAGE: 90-130kD, reducing conditions. Biological Activity: Measured by its ability to to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH. The specific activity is > 2250pmoles/min/ug Storage and Stability: For long-term storage, aliquot and store at -70°C. Aliquots are stable for 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, Chinese Hamster Ovary (CHO) cell line |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 78 |
Product Properties
| Storage | -70°C |
| Grade | Highly Purified |
| Purity | ≥90% (SDS-PAGE) under reducing conditions and visualized by silver stain Endotoxin: ≤1EU/ug (LAL) |
| Form | Supplied as a liquid in 25mM Tris base, 150mM sodium chloride, pH 7.5, 5umM ZnCl2 |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
