Endonuclease III, Recombinant, E. coli, aa1-211, His-Tag (nth, b1633, JW1625, DNA-(apurinic or apyrimidinic site) Lyase)
949.53 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-E2291-41C
Supplier
US Biologicals
Size
250 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Nth, also known as Endonuclease III, has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme incises damaged DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5′ from the damaged site. This enzyme is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. Description: Recombinant E. coli NTH (Endonuclease III) protein fused to His-tag at the N-terminus; expressed in E. coli AA Sequence: MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I Molecular Weight: 25.7kD (231aa) confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C or lower. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 25.7 |
Product Properties
| Storage | -20°C |
| Grade | Purified |
| Purity | ≥ 85% (SDS-PAGE) |
| Form | Supplied as a liquid in 20mM Tris-HCl buffer, pH8.0, 0.1M sodium chloride, 1mM DTT, 0.1mM PMSF, 40% glycerol. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
