Endoproteinase Asp N (Asp N Endopeptidase)
477.98 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-E2291-79
Supplier
US Biologicals
Size
2 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Endoproteinase Asp-N is a metallo endoprotease, isolated from a mutant strain of Pseudomonas fragi, which hydrolyzes peptide bonds on the N-terminal side of aspartic and cysteic acid residues. Endoproteinase Asp-N is HPLC purified, resulting in a product that is suitable for seuqencing analysis. In 100mM NH4HCO3, pH 8.5, or 100 mMTris HCl, pH 8.5, Asp-N specifically cleaves peptide bonds on the N-terminal side of aspartic and cysteic acid residues.. Self digestion may occur if temperatures above 37 °C are used. A known peptide such as glucagon should be run as a control for all experiments. Specific Activity: ≥ 20KU/mg Optimal pH: 6.0-8..5 Applications: Suitable for use in Sequencing. Storage and Stability: Lyophilized powder may be stored at 4°C. Reconstituted product is stable for several weeks at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Pseudomonas fragi mutant strain. |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 27 |
Product Properties
| Storage | 4°C/-20°C |
| Grade | |
| Purity | Homogenous on SDS-PAGE. MW: 27kD |
| Form | Supplied as a lyophilized powder in 10mM Tris HCl, pH 7.5. Reconstitute with 50ul sterile, ddH2O. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
