Enterokinase, Light Chain, Ultrapure, Recombinant, Human (Enteropeptidase)
434.83 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-E3300-01
Supplier
US Biologicals
Size
20u
Product Overview
Product Properties
Target Gene
Product Overview
| Description | A serine protease which cleaves proteins at the C-terminal side of Lysine after the sequence Asp-Asp-Aps-Aps-Lys; used to cleave recombinant fusion proteins which have the EK recognition sequence Glu4Lys or Asp4Lys. The light chain of enteropeptidase has full enzymatic activity. No other protease activity was detected. Human enteropeptidase binds specifically to STI-agarose. Unit Definition: One unit of human enteropeptidase will cleave 2mg of thioredoxin/human EGF fusion protein with the Asp-Asp-Aps-Aps-Lys sequence at the joining point in 22 hours at 4°C, in 16 hours at 25°C or in 8 hours at 37°C. Dilution Buffer: 50mM Tris-HCl, pH 8.0, 500mM sodium chloride, 1mM calcium chloride, 50% glycerol. Assay Conditions: 50mM Tris-HCl or sodium phosphate, pH 8.0 with or without calcium chloride. The enzyme is active at a pH range of 6.0-9.0. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 0.5M sodium chloride, 1mM calcium chloride, 50% glycerol. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
