Exonuclease III
421.97 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-E9008
Supplier
US Biologicals
Size
4000U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Exonuclease III is a 3’-5’ exonuclease releasing 5’ mononucleotides from dsDNA and exhibits AP-specific endonuclease, 3’ phosphatase and RNAse H activities. Source: E.coli cells carrying a cloned E.coli xth gene. Storage Buffer: Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 50mM KCl, 1mM DTT, 50% glycerol. Reaction Buffer (10X) E9008-05: Supplied as a liquid in 660mM Tris-HCl, pH 8.0, 6.6mM MgCl2 Unit Definition: One unit of Exonuclease III catalyzes the release of 1nmol of acid soluble reaction products from E. coli [3H]-DNA in 30 min at 37°C. Applications: Suitable for use in construction of nested unidirectional deletions of DNA fragments, generation of single-stranded template for DNA dideoxy sequencing, site-directed mutagenesis, cloning of PCR products and preparation of strand-specific probes. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 50mM KCl, 1mM DTT, 50% glycerol. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
