Fibrinogen, Plasminogen Depleted, Human (Coagulation Factor I)
248.84 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-F4200
Supplier
US Biologicals
Size
25 mg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Fibrinogen is an abundant plasma protein (5-10uM) produced in the liver. The intact protein has a molecular weight of 340kD and is composed of 3 pairs of disulfide-bound polypeptide chains named Aalpha, Bbeta and gamma. Fibrinogen is a triglobular protein consisting of a central E domain and terminal D domains. Proteolysis by thrombin results in release of Fibrinopeptide A (FPA, Aa1-16) followed by Fibrinopeptide B (FPB, Bb1-14) and the fibrin monomers that result polymerize in a half-overlap fashion to form insoluble fibrin fibrils. The chains of fibrin are referred to as a, b and g, due to the removal of FPA and FPB. The polymerized fibrin is subsequently stabilized by the transglutaminase activated Factor XIII that forms amide linkages between g chains and, to a lesser extent, a chains of the fibrin molecules. Proteolysis of fibrinogen by plasmin initially liberates C-terminal residues from the Aa chain to produce fragment X (intact DE- D, which is still clottable). Fragment X is further degraded to nonclottable fragments Y (D-E) and D. Fragment Y can be digested into its constituent D and E fragments. Digestion of non-crosslinked fibrin with plasmin is very similar to the digestion of fibrinogen, which results in production of fragments D and E. Degradation of crosslinked fibrin by plasmin results in fragment DD (D-Dimer consisting of the D domains of 2 fibrin molecules crosslinked via the y chains), fragment E (central E domain) as well as DDE in which fragment E is non-covalently associated with DD. For human crosslinked fibrin, the relative weights of the cleavage fragments produced are: 184kD for fragment DD, 92kD for D, 50kD for E, 1.54kD for FPA and 1.57kD for FPB. Applications: Suitable for use as an antigen in immunological protocols. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. Once thawed do not chill. Remaining protein may be aliquoted into a single use size and frozen. Aliquots are stable for 6 months. |
| Source Antigen | Human plasma |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 341 |
Product Properties
| Storage | -70°C |
| Grade | Purified |
| Purity | Homogeneous by SDS-PAGE and ≥95% clottable. Plasminogen has been depleted by affinity chromatography. |
| Form | Supplied as a liquid in 20mM sodium citrate-HCl buffer, pH 7.4. When thawing, warm to 37°C to assure complete protein solubility. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
