FLASH, CT, Blocking Peptide (FLICE-associated Huge Protein)
410.43 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-F4538P
Supplier
US Biologicals
Size
50 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Peptide (SERFQQLMKLFEKSKC) corresponding to amino acids 1253 to 1268 of human origin, which differ from those of mouse by one amino acid (1,2). A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2). FLASH is widely expressed. Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Positive Control: HeLa whole cell lysate Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Synthetic peptide |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Purified |
| Purity | Purified 60-70% |
| Form | Supplied as a lyophilized powder. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
