Growth Hormone, Chicken, Recombinant
419.70 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-G8989-95
Supplier
US Biologicals
Size
20 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Recombinant chicken growth hormone (cGH) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids with an additional Ala at its N-terminus and having a molecular mass of 22255 Dalton. cGH is purified by proprietary chromatographic techniques. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala. Dimers and aggregates: The purified cGH (22K) consists of > 99% monomers as determined by gel-filtration chromatography. Biological Activity: chGH is fully biologically active in homologous assays and in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. Endotoxin: Less than 0.1ng/ug (IEU/ug) of cGH (22 K). Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.75 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of others GHs as a Reference Standard. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O or buffer of choice and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 22.26 |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ≥ 98% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel. (c) Analysis by RP-HPLC. |
| Form | Supplied as a lyophilized powder from 0.3% NaHCO3 adjusted to pH 8. Reconstitution: It is recommended to reconstitute the lyophilized cGH in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml and not more than 3mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
