IDH1, Recombinant, Human, FLAG-Tag (Isocitrate Dehydrogenase [NADP] Cytoplasmic, IDH, Cytosolic NADP-isocitrate Dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase, PICD)
660.43 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-137303
Supplier
US Biologicals
Size
50 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Description: Recombinant human IDH1 (aa 1-414, GenBank accesssion #NM_005896; UniProt O75874) with C-terminal FLAG tag, expressed in Sf9 insect cells via a baculovirus expression system. Molecular Weight: ~47.6kD Specific Activity: ≥5600pmol/min/ug Assay Conditions: Reaction was conducted in 50mM Tris (pH 7.4), 10mM MgCl2, 200uM DL-isocitrate, and 200uM NADP. Incubation was carried out for 30 minutes at room temperature. Absorbance was read at 340nm using Tecan Infinite 1000 (5 flash/10ms settle time). Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Source Antigen | Sf9 insect cells |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 47.6 |
Product Properties
| Storage | -70°C |
| Grade | Purified |
| Purity | Purified (~80%) |
| Form | Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 90ug/ml FLAG peptide, 3mM DTT, 10% glycerol. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
