IRAK4, Recombinant, Human, GST-Tag (IRAK4, REN64, NY-REN-64, Interleukin-1 receptor-associated kinase 4, IRAK-4 mutated form 1, Interleukin-1 receptor associated kinase 4)
701.54 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-I8600-17H
Supplier
US Biologicals
Size
10 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | (IRAKs) are important mediators in the signal transduction of the Toll/IL-1 receptor (TIR) family. Members of the TIL family play a role in innate immune responses by coordinating host defense mechanisms. IRAK4 is the most recently discovered IRAK protein. IRAK-4 has been shown to interact with IRAK-1 and TRAF6 in an IL-1-dependent manner, and overexpression of IRAK-4 has been shown to activate NFkB as well as mitogen-activated protein (MAP) kinase pathways. Studies demonstrate that mice lacking IRAK4 are severely impaired in their IL-1, Toll-like receptor (TLR) signaling and response to viral and bacterial challenges; this indicates that IRAK4 has an important role in innate immunity. Humans lacking IRAK 4 exhibit pyogenic bacterial infections, while their blood and fibroblast cells fail to activate NF-kB. IRAK-4 is believed to be required for the LPS-induced activation of antigenpresenting cells. Source: Recombinant human IRAK4, aa2-end, with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system. Molecular Weight: 77.5kD Specific Activity: 18pmol/min/ug Reaction Buffer: 50mM HEPES (pH7.5), 10mM MgCl2, 1mM EGTA, 200uM ATP, 0.01% Brij-35 and 2uM substrate. Substrate: Invitrogen Z-Lyte SER/THR 7 Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: The enzyme reaction was carried out for 1h at room temperature in reaction buffer. Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. |
| Source Antigen | Recombinant, Baculovirus infected Sf9 cells |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 77.5 |
Product Properties
| Storage | -70°C |
| Grade | Purified |
| Purity | ≥67% |
| Form | Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 400mM NaCl, 10% glycerol, 3mM DTT. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
