Lactate Dehydrogenase (LDH), Total discontinued
542.89 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-143072
Supplier
US Biologicals
Size
100U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36kD subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H)-in the heart, LDH-2 (3H1M)-in the reticuloendothelial system, LDH-3 (2H2M)-in the lungs, LDH-4 (1H3M)-in the kidneys and LDH-5 (4M) in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a flipped pattern) suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization. Total Protein Per Vial: As reported Specific Activity (units per mg protein): As reported. Activity, units per mg product (measured using Dimension Clinical Chemistry system): As reported. Each vial contains a total of 100U of activity. Unit Definition of Activity: One unit will catalyze the oxidation of 1 micromole of L-lactate to pyruvate per minute at 37°C, pH 8.55. Isoenzyme Electrophoresis: LD1: 37% LD2: 26% LD3: 29% LD4: 4% LD5: 4% Note: These values are representative of one lot. The isoenzyme distribution will exhibit lot-specific variations. Contaminants: CK: <0.1% GOT: <0.1% Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer prior to use. Aliquot unused portion qnd store at -20°C. to avoid repeated freezing and thawing and Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Human heart |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | Highly purified |
| Form | Supplied as a powder lyophilized in 20mM Tris chloride, pH 7.5, 1mM DTT, 1mM EDTA. Reconstitute to 1mg/ml in the same buffer. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
