MssI (PmeI)
406.91 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-M4692-60
Supplier
US Biologicals
Size
250U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | 5′-G T T T^A A A C-3′ 3′-C A A A^T T T G-5′ Concentration: 5u/ul Source: Methylobacterium species Dd 5–732 Unit Definition: One unit is defined as the amount of Mssl required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. Diluent Buffer : 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl, pH 7.5, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Supplied with: R1625-Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate (pH 7.9 at 37°C), 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. R1625-04 Restriction Enzyme Buffer E, 10X: Dilute to 1X for 100% Mssl digestion. 10mM Tris-HCl, pH 7.5, 10mM MgCl2 and 0.1mg/ml BSA. Incubation Temperature: 37°C Storage and Stability: Aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Methylobacterium species Dd 5–732 |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
