MvaI (EcoRII*)
403.54 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-M9580
Supplier
US Biologicals
Size
2000U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | 5′-C C^A G G-3′ 3′-G G T^C C-5′ NOTE: MvaI-neoschizomer of EcoRII, produces DNA fragments that have a 1-base 5′-extension. Unlike EcoRII, MvaI is not blocked by Dcm methylation. Source: E.coli that carries the cloned mvaIR gene from Micrococcus varians RFL19 Concentration: 10u/ul Unit Definition: One unit is defined as the amount of M9580 required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. Form: Supplied in 10mM Tris-HCl, pH 7.5, 400mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Supplied With: R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA (pH 7.9 at 37°C). R1625-04: Restriction Enzyme Buffer E, 10X: Supplied as a liquid in 10mM Tris-HCl, pH 8.5, 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 37°C. Dilute with: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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| Reactivity | |
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| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
