NheI
437.11 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-N2425
Supplier
US Biologicals
Size
1000U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | 5′-G^C T A G C-3′ 3′-C G A T C^G-5′ Source: Neisseria mucosa heidelbergensis Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA-HindIII fragments in 1 hour at 37°C in 50ul of assay buffer. Concentration: 10 units/ul Supplied With: R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA (pH 7.9 at 37°C) when diluted to 1X. Diluent Buffer: 10mM Tris-HCl, (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Storage Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 8.0 at 25ºC, 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Incubation Temp: 37°C Compatible Ends: BcuI, Eco130I, XbaI, XmaJI Number of Recognition Sites in DNA Molecules: Lambda: 1 PhiX174: 0 M13mp18/19:0 pBR322: 1 pUC18/19: 0 pUC57: 0 pTZ19R/U: 0 *Note: Supercoiled plasmids may require up to 10-fold more Nhel for complete digestion than linear DNAs (e.g. 10units are required to cleave 1ug of pBR322 DNA). Thermal Inactivation: NheI is inactivated by incubation at 65°C for 20min. Stability During Prolonged Incubation: ≥ 0.2units of NheI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37ºC. Enzyme Properties: Methylation Effects on Digestion: Dam, Dcm, EcoKl, EcoBl: never overlaps – no effect CpG: May overlap – cleavage impaired Digestion of Agarose-embedded DNA: A minimum of 5 units of Nhel is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16hrs |
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| Reactivity | |
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Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
