Noggin, Recombinant, Mouse
679.46 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-215049
Supplier
US Biologicals
Size
20 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Noggin is a secreted polypeptide, originally identified as an antagonist of BMP-4, which binds to and inactivates signal transduction by members of the transforming growth factor-beta (TGF-beta) superfamily, and plays an essential role in cartilage morphogenesis, joint formation and neural tube fusion, as determined by mouse knockout studies. Noggin is implicated in both early and later developmental stages, and can be used as a tool to differentiate human embryonic stem cells into neural precursor cells. Source: Recombinant protein corresponding to mouse Noggin expressed in E. coli. Molecular Weight: ~46.4kD (206aa) Activity: Determined by its ability to inhibit ~5ng/ml of BMP-4 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is ~1-2ng/ml of Noggin Applications: Suitable for use in Functional Assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Endotoxin Level: <1EU/ug Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 46.4 |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ~95% (SDS-PAGE, HPLC) |
| Form | Supplied as a lyophilized powder. No preservative added. Reconstitute with 200ul sterile ddH2O. Further dilutions can be made in H2O, 0.1% BSA. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
