Nucleosome, Recombinant, Human (Biotin)
894.28 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-171938
Supplier
US Biologicals
Size
20 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Human recombinant nucleosomes consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A (a.a.2-130(end)), H2B (a.a.2-126(end)), H3 (a.a.2-137(end)), and H4 (a.a.2-103(end)), expressed in an E. coli expression system. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Each histone protein has an N-terminal His-tag each H2B has a C-terminal Cys added as a biotinylation site.Total histone MW (before biotinylation) =113.8kD. Source: Recombinant protein corresponding to aa2-137 of Nucleosome at the N-terminus, expressed in E.coli. Molecular Weight: ~113.8kD Application: Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap |
| Source Antigen | Recombinant, E.coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 113.8 |
Product Properties
| Storage | -70°C |
| Grade | Highly Purified |
| Purity | Highly Purified (~99%) |
| Form | Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 1mM DTT, 20% glycerol. Labeled with Biotin. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
