PaeI (SphI)
447.24 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-P1800
Supplier
US Biologicals
Size
500U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Sequence: 5′-G C A T G^C-3′ 3′-C^G T A C G-5′ Source: Pseudomonas aeruginosa Concentration: 10u/ul Unit Definition: One unit is defined as the amount of PaeI required to digest 1ug of lambda DNA fragments in 1 hr at 37°C in 50ul assay buffer. Diluent Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Storage Buffer: 10mM potassium phosphate (pH 7.0 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA, 0.15% Triton X-100 and 50% glycerol. Supplied with: R1625: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA, pH 7.9 at 37ºC. R1625-01: Restriction Enzyme Buffer B, 10X: Supplied as a liquid 10mM Tris-HCl, pH 7.5 at 37°C, 10mM magnesium chloride, 0.1mg/ml BSA. Incubate at 37°C Double Digests: R1625 is provided to simplify buffer selection for double digests. 98% of PaeI, works well in a 1x or 2x. |
| Source Antigen | |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
