Papain, Suspension
387.35 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-P3100-02
Supplier
US Biologicals
Size
25 mg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Proteolytic enzymes are widely used in cell isolation. With some tissues papain has proved less damaging and more effective than other proteases. Papain is a sulfhydryl protease from Carica papaya latex. Native crystalline papain is quite unreactive until acted upon by mild reducing agents such as cysteine or cyanide. It may exist as a zymogen (Brocklehurst and Kierstan, 1973). For a general review, see Liener (1974). Papain has wide specificity. It will degrade most protein substrates more extensively than the pancreatic proteases. It is also an esterase. The action of papain on leucine methyl ester produces an insoluble polyleucine peptide. Papain breaks down the intercellular matrix of cartilage. Papain is a single peptide chain of 211 residues folded into two parts that form a cleft. A three-dimensional structure has been indicated. The molecule has one free SH group which is functional. There are seven subsites, each is capable of accommodating a single amino acid residue of a peptide substrate. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap |
| Source Antigen | Carica Papaya Latex |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 38.9 |
Product Properties
| Storage | 4°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | Supplied as a 2X crystalline suspension in 0.05M sodium acetate, pH 4.5 |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
