Parvalbumin, Recombinant, Rat
419.70 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-P3113-52
Supplier
US Biologicals
Size
2 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Parvalbumin (PV or PVALB or oncomodulin) is a high affinity low mol wt (~12kD) cytoplasmic protein belonging to the troponin C superfamily. It is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. PV has two isoform: alpha (rat/mouse/human 110-aa, 7p13-p11) beta form (mouse/rat/human 109-aa) shares approx. 48% identity (mouse PVs). The PV-alpha is less acidic (pI=5) than the Beta-isoform and has an additional amino acid in the C-terminal helix. PV-alpha is also expressed in inner ear. PV-beta isoform is expressed in many fetal tissues, placenta and tumors. Applications: The Parvalbumin can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For fluid-phase adsorption in immunohistochemistry we suggest the following procedure: A- Dilute 1 µl of the mon oclonal antibody 235 (Swant) against parvalbumin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5000). B- Add 1µg of the Parvalbumin to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex,DAB). As a result, the immunostaining should be strongly reduced or even completely prevented. Solubility: It is recommended to reconstitute the lyophilized Parvalbumin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Stability: Lyophilized Parvalbumin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Parvalbumin should be stored at 4°C between 2-7 days and for future use below –18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ≥ 90% as determined by reducing and non-reducing SDS-PAGE Coomassie. |
| Form | Supplied as a lyophilized powder in 10mM Tris-base, 70mM sodium chloride, 1mM EDTA and 1mM beta-mercaptoethanol, pH 7.4. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
