PdiI (NaeI)
398.88 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-P3121
Supplier
US Biologicals
Size
200U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | 5′-G C C^G G C-3′ 3′-C G G^C C G-5′ Concentration: 10u/ul Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9 at 37°C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Diluent Buffer: 10mM Tris-HCl, pH, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Storage Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 500mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 0.15% Triton X-100, 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with P3121. Ligation and Recleavage (L/R) Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test abililty to trace nuclease and phophatase activities with senstivity that is higher than L/R by a fctor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide occurred during incubation with 10 units of Pdil for 4 hours. Blue/White Cloning Assay: The B/W assay was replaced with L0 test after validating experiments showed L0 test ability to detect nuclease and phophatase activities with sensitivity that equals to that of B/W test. Methylation Effects: No effect on Dam, Dcm, EcoKl and EcoBl. Overlaps and blocks CpG. |
| Source Antigen | Pseudomonas diminuta Mck 33–321 |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
