Pectolyase discontinued. See P3130
1,196.65 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-474339
Supplier
US Biologicals
Size
1g
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Pectolyase contains two types of pectinase, endopolygalacturonase (E.C.3.2.1.15), endo-pectin lyase (E.C.4.2.2.3) and a maceration stimulating factor. Pectolyase is used in plant protoplast preparation to digest cell wall prior to organelle isolation. Purified pectinase is a multi-component preparation highly effective in depolymerizing plant pectins with varying degrees of esterification. Important enzymatic activities include pectin lyase (EC 4.2.2.10) and activities on polygalacturonic acid (pectin) and nonmethylated polygalacturonic acid (pectate). The product contains substantial hemicellulase, cellulase, pectinesterase and xylanase activities which together with pectin lyase and polygalacturonase work synergistically to digest plant cell wall tissues. CAS No: 9033-35-6 Molecular Weight: 373.90 Source: Aspergillus japonicus Appearance: Supplied as a brownish powder. Specific Activity: ≥1000 units per gram (pectin lyase) Unit Definition: One unit will liberate 1umole of galacturonic acid from polygalacturonic acid per minute at pH 5.5 at 25°C. Assay Principle: Pectin—-pectin lyase—->Oligosaccharides with terminal 4-deoxy-6-methoxy-a-D-galact-4-enuronosyl groups. Enzymatic Reactions, Major: Pectin Lyase (4.2.2.10) Polygalacturonidase (3.2.1.15) Galacturan 1,4-α-galacturonidase (3.2.1.67) Enzymatic Reactions, Minor: Cellulase (Endocellulase (3.2.1.4), Exocellulase (3.2.1.91), Cellobiase (3.2.1.21)) Hemicellulase (3.1.1.73) Pectinesterase (3.1.1.11) Xylanase (3.2.1.8) pH: 4.5-6.5 (effective range); 5.5 (optimum); 4.0-7.0 (stable range) Loss on Drying: ≤5% Solubility: Soluble in water Storage and Stability: Store at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. |
| Source Antigen | Aspergillus japonicus |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 373.9 |
Product Properties
| Storage | 4°C |
| Grade | Molecular Biology Grade |
| Purity | Specific Activity: ≥1000 units per gram (pectin lyase) |
| Form | Supplied as a brownish powder. |
| Reactivity Life | |
| Note | For reserch purpose only |
Target Gene
NA
