Period Clock Protein 1, Mouse, Control Peptide (PER1, RIGUI)
465.58 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-P3342-12A
Supplier
US Biologicals
Size
100 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Control Peptide for P3342-11 (affinity purified antibody) and P3342-01C (antiserum). Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms. In Drosophila, the genes period (dPer) and timeless (tim), and in Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm. Recently human and mouse genes encoding a basichelix-loop-helix (bHLH) and Per-ARNT-Sim (PAS)-domain with significant similarity to the Drosophila Period have been reported. The cDNA sequences of hPER and mPer1 (also named RIGUI) are predicted to encode for proteins of length 1290 and 1291 amino acids respectively. Homologues of mPer1 designated Per 2 (1257aa) and Per3 (1113aa) have also been cloned. Both Per1 and Per2 levels show circadian rhythm in the SCN and eyes. It has been suggested that mPer regulates neuronal activity in the SCN. Using genetic approach, a single mutation (A to T in the Clock gene affects circadian rhythmicity in mice. Clock has been mapped to chromosome 5. Mouse Clock encodes a transcription factor, a single polypeptide chain of 855aa (predicted calculated mol wt ~97kD; pI 6.52; hClock, 846aa). Clock is abundantly expressed in brain (SCN, pyrifom cortex, hippocampus) as well as in other tissues (eye, total brain, tested, ovaries, liver, heart, lung, and kidney). Although, Clock is constitutively expressed (not rhythmic) in the SCN, it may still be an important component of circadian machinery. Applications: Suitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilution: ELISA: 50-100ng of control peptide/well. Antibody Blocking: 5-10ug per 1ug of P3342-11 (affinity purified antibody) or 1ul P3342-01C (antiserum). Optimal dilutions to be determined by the researcher. Control Peptide: P3342-12A Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Mouse synthetic peptide |
| Reactivity | A 16-aa peptide internal sequence mapping downstream of the SG repeat of mouse Per 1. No significant homology is seen with Per2, Per 3, dPER or other known proteins. Species homology: human Per1 (93%). |
| Cross Reactivity | Human Mouse |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | Chromatographically purified |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
