PGAM1, Recombinant, Human, aa1-254, His-Tag (Phosphoglycerate Mutase 1)
460.56 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-369110
Supplier
US Biologicals
Size
20 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in this protein cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Source: Recombinant protein corresponding to aa1-254 from human PGAM, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~30.9 kD (274aa), confirmed by MALDI-TOF AA Sequence: MGSSHHHHHH SSGLVPRGSH MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK Specific Activity: >300units/mg, in which one unit will convert 1umole of 3-phosphoglycerate to 2-phosphoglcerate/minute at pH 7.6 at 37°C. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, human from E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 30.9 |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ~95% (SDS-PAGE) |
| Form | Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 10% glycerol. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
