Phosphodiesterase 1
451.93 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-P4072
Supplier
US Biologicals
Size
100u
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5′-mononucleotides from 3′-hydroxy-terminated ribo-and deoxyribo-oligonucleotides. The enzyme has been widely utilized as a tool for structural and sequence studies of nucleic acids. The enzyme has been purified with endonuclease activity being eliminated as well as 5′-nucleotidase and nonspecific monophosphatase. It is nonspecific with respect to base or sugar moieties of nucleotides. A variety of synthetic substrates are hydrolyzed. The exonuclease will not recognize nucleoside units in the syn conformation. ADP-ribosylated proteins are cleaved at the pyrophosphate linkages by venom phosphodiesterase to yield phosphoribosyl-AMP. Activity: ≥20 units per mg dry weight Absorbance (A280): As reported Unit Definition: 1 unit hydrolyzes 1 umole of p-nitrophenyl thymidine-5-phosphate per minute at 25ºC, pH 8.9. Nucleotidase: ≤8% Optimum pH: 9.8-10.4 Inhibitors: Reducing agents such as glutathione, cysteine and ascorbic acid. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. Activators: The enzyme has an absolute requirement for Mg2+ indicates an optimum concentration of 15mM. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Crotalus adamanteus venom (Eastern diamondback rattlesnake) |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 115 |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | Supplied as a lyophilized powder |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
