Plasminogen, Human, 95% (Profibrinolysin)
555.20 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-P4256-35
Supplier
US Biologicals
Size
1 mg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Plasminogen is a single chain glycoprotein zymogen which is synthesized in the liver and circulates in plasma at a concentration of approximately 2.4mM (1,2). The plasminogen molecule contains 790 amino acids, 24 disulfide bridges, no free sulfhydryls and 5 regions of internal sequence homology, known as kringles, between Lys77 and Arg560. These five triple-looped, three disulfide bridged, kringle regions are homologous to the kringle domains in t-PA, u-PA and prothrombin. Plasminogen contains one high affinity (Kd=9x10e-6M) and four low affinity (Kd=5x10e-3M) lysine binding sites. The high affinity binding site resides within the first kringle region of plasminogen. The interaction of plasminogen with fibrin and a2-antiplasmin is mediated by these lysine binding sites. Native glu-plasminogen (Mr=88,000) is readily converted to Lys-77-plasminogen (Mr=83,000) by plasmin hydrolysis of the Lys76-Lys77 peptide bond. Elastase catalyzed cleavage of the Val441-Val442 peptide bond of glu-plasminogen yields a functionally active zymogen termed Val-442 plasminogen or mini-plasminogen. The conversion of plasminogen to plasmin occurs by a variety of mechanisms, but all result in hydrolysis of the Arg560-Val561 peptide bond of plasminogen, yielding two chains which remain covalently associated by a disulfide bond. Activity: ≥120 units per mg protein. One unit is defined as the amount of enzyme, which will hydrolyze 1micromole of N-tosyl-L-arginine ester in 30 minutes at 37° C at pH 8.0. Molecular Weight: ~90kD Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Human serum or plasma |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 90 |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ≥95% by SDS-PAGE |
| Form | Supplied as a lyophilized powder from 20mM Tris, pH 7.5, 2mM EDTA. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
